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KMID : 0613820120220050627
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Journal of Life Science
2012 Volume.22 No. 5 p.627 ~ p.633
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Biological Analysis of Enzymatic Extracts from Capsosiphon Fulvescens Using the Microbulbifer sp. AJ-3 Marine Bacterium
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Bae Jeong-Mi
Cho Eun-Kyung
Kim Hye-Youn
Kang Su-Hee
Choi Young-Ju
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Abstract
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Microbulbifer sp. AJ-3 was used to acquire the degrading products from Capsosiphon fulvescens (DPCF), which were investigated to determine its physiological activities. A crude enzyme extract from Microbulbifer sp. AJ-3 hydrolyzes polysaccharide substrates such as agar, agarose, alginic acid, fucoidan, laminaran, starch, and chitin. Among them, agarose, laminaran, and alginic acid showed higher activities, especially alginic acid. The antioxidant activity of DPCF was measured by using 2,2-diphenyl-1-picryl-hydrazyl (DPPH) and superoxide dismutase (SOD)-like activities and were about 32% and 93% at 2 mg/ml, respectively. In addition, the nitrite-scavenging activity of DPCF was about 82%, 53%, and 12% at pH levels of 1.2, 3.0, and 6.0, respectively. To determine the influence of DPCF on alcohol metabolism, the generating activity of reduced-nicotinamide adenine dinucleotide (NADH) by alcohol dehydrogenase (ADH) was measured. The facilitating rate of ADH activity by DPCF was 130% at 2 mg/ml. The tyrosinase inhibitory activity of DPCF was slightly increased in a dose-dependent manner and was about 28% at 2 mg/ml. These results indicated that the enzymatic products from DPCF have a strong antioxidant, nitrite scavenging, and alcohol metabolizing activity.
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KEYWORD
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Capsosiphon fulvescens, polysaccharide-degrading enzymes, enzymatic hydrolysis, antioxidative activity, ADH activity
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